Journal: Nature Communications

Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply

doi: 10.1038/s41467-018-03775-y

Figure Lengend Snippet: Anemia in apoc2 mutant zebrafish. a Wright–Giemsa (Hema) and o-dianisine staining of peripheral blood cells from adult (18–20-month-old) male wild-type (WT) and apoc2 mutant zebrafish. b Quantitative results of peripheral blood cell count ( n = 11 for WT and n = 9 for apoc2 mutant groups). c Representative bright field images and quantitative results of blood cell (yellow arrows) count in the caudal vein (outlined with white dashed lines) of WT and apoc2 mutants at 52 hpf ( n = 8 in WT and n = 9 in apoc2 mutant groups) and 6.3 dpf ( n = 11 for WT and n = 13 for apoc2 mutant groups). See also Supplementary Movies and 2. d Wright–Giemsa staining of blood smears from WT and apoc2 mutants at 52 hpf and 6.3 dpf. Immature erythrocytes containing larger and less condensed nuclei are indicated with red arrows in a and d . Immature erythrocytes with weaker hemoglobin staining are indicated with green arrows in a . e o-Dianisine staining of 52 hpf and 6.3 dpf WT and apoc2 mutant embryos. f Effect of lomitapide: WT and apoc2 mutants were treated with 5 μM lomitapide starting from 2 dpf until embryos were fixed at 6 dpf for ORO staining. Black arrows point to intestinal lipid accumulation and green arrows to circulating lipids. g Blood cell counts in the caudal vein of WT, apoc2 mutants and apoc2 mutants treated with lomitapide at 6.3 dpf ( n = 5 in each group). h Blood cell counts in the caudal vein of 14 dpf WT, apoc2 mutants fed with normal diet and apoc2 mutants fed with low-fat diet (LFD) starting at 5 dpf ( n = 4 in WT and apoc2 mut groups each; n = 5 in apoc2 + LFD group). Scale bars, 20 μm in a , c , and d , 100 μm in e , and 200 μm in f . Mean ± SEM; *** P < 0.001 (Student’s t test)

Article Snippet: Bone marrow cells were collected from mouse femur and tibia and depleted from red blood cells with a red blood cell lysis buffer (eBioscience, Cat. 00–4333).

Techniques: Mutagenesis, Staining, Cell Counting